Bleach V Menu V2 [UPDATED]
Disinfectants, bleach, and soap and water may be used to clean surfaces, an important step in stopping the spread of the coronavirus that causes COVID-19. Never attempt to self-treat or prevent COVID-19 by rubbing or bathing anywhere on your body with bleach, disinfectants or rubbing alcohol. Effective hand sanitizers do contain alcohol, but they are formulated to be safe for use on hands.
Bleach V Menu v2
1) I'd like to +1 on request to add (at least an option) to add bleach bit "shred files" to context menu ( +bug/1654449).There aren't many / any? file shredders for Linux ** with a GUI or context menu option ** - unless someone has found any.
3) For now, a "quick launch" icon can be added to Linux's panel. But if every program or function that's used from context menu instead was on the quick launch / panel, there'd be no room for running apps icons on the panel or taskbar.
5) Re: BB shredding buttons labeling inconsistency. On main menu, it's "Shred Files (Folders)."But the browse to file screen shows "Delete" on the button -not Shred (not the same & a little misleading to some). Same for confirmation screen button. Dialog says "are you sure you want to permanently delete these files?," instead of shred or erase.
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The simple ratio method seems to be the most widely used method. It has been applied for improving the deconvolution results in 3D stacks7 and in the fluorescence recovery after the photobleaching (FRAP) technique. In FRAP literature, this method is called double normalization2,3. In ImageJ, this method was implemented as a macro by Jens Rietdorf and has been available since 2004 ( _correction.html).
In the exponential fitting method, it also compensates the loss by multiplying the inverse of the bleach ratio, but the method first fits the exponential equation to the bleaching curve and uses the fitted parameters for deriving the bleach ratio at each time point8,9. This method has been implemented as an ImageJ plugin and is available as a part of MBF ImageJ for Microscopy bundle ( _Plugin_Collection). A plugin PixBleach allows three different exponential decay models to be used for the correction ( )10.
The exponential fitting method has an assumption that the bleaching process follows a single exponential decay. It is known that in some cases bleaching time course is a double exponential decay8. In this respect, one must consider before using this method if there is any good reason to model the bleaching process of the sample as a single exponential decay. Otherwise, the goodness of fit needs to be evaluated for the proper use of this method. In addition, the exponential fitting method ignores small perturbations in the intensity such as abrupt changes in the emission of fluorescence. Such changes can be caused by small fluctuations in the power of the excitation light or in the slight variations in the timings of the shutter controlling the light path. The simple ratio method deals better with such changes. However, such a non-flexible nature of the exponential fitting method can become an advantage in some other occasions. For example, if the change in the intensity is due to the synthesis of GFP molecules by cell, the simple ratio method will wrongly correct such true increase and mask the biological event, but the exponential fitting method will achieve a better correction as long as the bleaching is known to be a single exponential decay.
Without the black lines, all four panels can be merged into one panel, with different methods in different colors. Doing so will free up room for a few other bleaching correction examples. Ideally, such examples would reflect cases that highlight the positive/negative sides of each method. In my opinion that would really elevate the paper beyond a fancy manual. (I have imaged some data for this review that the author is free to use for this purpose, if desired.).
I have reproduced the bleaching corrected data with the sample image used in the article, but now also separated the foreground (the cell) from background (using a simple Otsu threshold on a slice where the cell is in focus, halfway the time-lapse). The resulting time traces (total, cell, background) show that when the background set at 64, the background intensityincreases by 45% while the cell intensitydecreases by 35%: under-correction. With background at 72 the background intensitydecreases by 42%, while the cell intensityincreases by 29%: over-correction. (In the latter case the background gray values are almost zero everywhere, because the image remains 8-bit; this could be part of the problem.)
Structure of the article. Methods and results could be fused, such that the explanation of a method goes directly along with presentation of the correction result on the sample data set. It should be referred to Fig 1 earlier in the text, e.g. Fig 1 top left to illustrate bleaching when introducing it in the very beginning. The chosen structure could be due to guidelines of the publisher and in this case it should be communicated to the publisher.
Since the advent of using clay to adsorb color pigments and impurities from edible oil, processors have been confronted with the problem of spent earth disposal. Typically, spent bleaching earth contains entrained oil in the range of 30 to 50% by weight. The entrained oil is in the form of a thin film spread over the immense surface area provided by the clay particles. Upon exposure to air, rapid oxidation of the oil film occurs and sufficient heat is generated to cause spontaneous combustion. Consequently, the most common method of spent earth disposal is to haul and bury it at a solid waste disposal site. Handling and disposing of spent earth is a fire risk, an operating expense, and a source of environmental regulatory concerns. In addition, the value of the oil that is lost is a significant cost.
For more than 100 years, ideas about practical uses for spent bleaching earth have been originated and tried without much success. Integrated oilseed processors and edible oil refiners have had limited success in adding some spent bleaching earth to meal by taking advantage of the allowable use of clay as a flow aid. Due to problems involving safe handling and uniform blending, the addition-to-meal practice is limited. There is some adding of spent bleaching earth to livestock feed in several countries, but the risk of spontaneous combustion is typically a barrier.
In 2011, the author originated the idea that salt could be added and mixed with spent bleaching earth to eliminate the spontaneous combustion problem and provide a high-demand salt-lick product for livestock. A provisional patent was filed to protect the idea and to provide the time for validation. During the summer of 2012, experimental work was conducted to verify that the hydroscopic properties of salt, together with the dilution effect, would eliminate the spontaneous combustion problem with spent bleaching earth. The experimental work was carried out over a week using spent bleaching earth that came directly from an edible oil processing plant (Fig. 1). It was found that salt content as low as 35% would eliminate spontaneous combustion. To provide an additional safety factor, 45% salt addition was established as the minimum.
The formulation of salt-lick products that incorporates spent bleaching earth has a broad range of possibilities. An array of minerals can be added on the basis of consumer preference. Some amount of protein meal and/or other feed ingredients can be added if that is the consumer preference.
For livestock, salt is supplied in solid blocks, pellets, and fine particles. From the perspective of supply, it would be desirable to supply the spent bleaching earth and salt mixture for livestock-lick products in all three forms. A literature search was made to determine what bonding agents have been commonly used to make solid blocks for livestock feed. It was found that Type I/II Portland cement has been used for that purpose in making a variety of livestock feed blocks in several countries. Given that Type I/II Portland cement contains about 75% calcium minerals, it is beneficial for livestock ingestion. For the solid block and pellet product, Portland cement was added at levels of 5, 10, and 15%. For the fine particle or granular product, a mixture of spent bleaching earth and salt is acceptable. However, adding 5% Portland cement as a desiccant and mineral agent prevents lumping during long-term storage.
Considering cattle alone, if all of the spent bleaching produced per year in the United States was used to make salt-lick products, it would satisfy only about 10% of the demand for salt based on 20 pounds of salt per animal. Taking into account the salt needs of cattle, sheep, goats, hogs, and horses worldwide, the available market overwhelms the potential supply.
On the basis of producing product in 50-pound units that include 35% spent bleaching earth by weight, 35% of the product can be produced at no cost to an edible oil processor. If the spent bleaching earth is 40% edible oil by weight, 7.0 pounds of edible oil is contained in each unit. Using a five-year average price for yellow grease (fat applicable to animal feed) of $0.30 per pound, the value of the edible oil in each 50-pound unit is $2.10. If the all the other ingredients were priced at purchase cost plus the cost of each unit production, the value (profit potential) of each pound of spent bleaching earth would be $2.10 per 50-pound unit.
The scheme for producing an array of salt-lick products from spent bleaching earth is quite simple and involves standard equipment used in the livestock feed industry. Diagramed in Fig. 4, the batch production process involves the following sequence for adding ingredients to the product mixer: 041b061a72